New Directions in Electrophoretic Methods by James W. Jorgenson and Marshall Phillips (Eds.)

By James W. Jorgenson and Marshall Phillips (Eds.)

content material: evaluate of electrophoresis / James W. Jorgenson --
Electrophoresis in polyacrylamide gels / Michael J. Dunn --
Immobilized pH gradients : contemporary advancements / Pier Giorgio Righetti, Cecilia Gelfi, and Elisabetta Gianazza --
Rehydratable polyacrylamide gels for ultrathin-layer isoelectric focusing / Bertold J. Radola --
Silver-stain detection of proteins separated via polyacrylamide gel electrophoresis / Carl R. Merril --
colour silver staining of polypeptides in polyacrylamide gels / David W. Sammons and Lonnie Adams --
improvement of electrophoresis and electrofocusing criteria / Dennis J. Reeder --
Standardization in isoelectric concentrating on ultrathin-layer rehydratable polyacrylamide gels / Robert C. Allen and Peter M. Lack --
software of high-resolution, two-dimensional electrophoresis to the research of wheat, milk, and different agricultural items / Norman G. Anderson and N. Leigh Anderson --
functions of isoelectric focusing in forensic serology / Bruce Budowle and Randall S. Murch --
Biophysical characterization through agarose gel electrophoresis / Philip Serwer --
Pulsed electrophoresis / David C. Schwartz --
Capillary quarter electrophoresis / James W. Jorgenson --
Capillary isotachophoresis / F.M. Everaerts and Th. P.E.M. Verheggen --
Isotachophoresis of man-made ion-containing polymers / L. Ronald Whitlock --
contemporary advances in preparative electrophoresis / Richard A. Mosher, Wolfgang Thormann, Ned B. Egen, Pascal Couasnon, and David W. Sammons.

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Cremoris i n a pH 4-8 IPG. (A) 3%T, 4%C polyacrylamide g e l containing 8M urea and 2% NP-40. 0. (B) same as i n (A) but with sample and g e l containing 4% Ampholine pH 4-8 and no urea. (C) pattern of focused c a r r i e r ampholytes as i n (B) v i s u a l i z e d by complexing with Pb++ and p r e c i p i t a t i n g i n s i t u PbS. A l l gels were focused f o r 30,000 Vh at 10*C (Reproduced with permission from Ref. 35. Copyright 1985, Verlag Chemie). ; ACS Symposium Series; American Chemical Society: Washington, DC, 1987.

Literature Cited 1. 2. 3. 4. ; Weintraub, L. Science 1959, 130, 711-713. Ornstein, L. Ann. Y. Acad. Sci. 1964, 121, 321-349. J. Ann. Y. Acad. Sci. 1964, 121, 404-427. ; Rickwood, D. Eds ; Gel Electrophoresis of Proteins; IRL Press: Oxford, 1981. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1987. 2. DUNN 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. G. "Isoelectric focusing: theory, methodology and applications"; Elsevier: Amsterdam, 1983.

Protides Biol. Fluids 1985, 33, 439-442. 64. ; Rossman, U. Electrophoresis 1985, 6, 314-325. 65. G. Electrophoresis 1985, 6, 419-422. 66. D. Nature 1956, 177, 1033-1034. 67. H. J. Biol. Chem. 1975, 250, 4007-4021. 68. Klose, J . Humangenetik 1975, 26, 231-243. 69. Klose, J . J. ; G. Thieme: Stuttgart, 1975, pp 375-387. 70. A. J . Biol. Chem. 1975, 250, 5375-5385. 71. M. Electrophoresis 1983, 4, 173-189. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1987. 2. DUNN 72. 73. 74.

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