Iron Metabolism: Diagnosis and Therapy of Anemias by Dr. Manfred Wick, Prim. Univ.-Prof. Dr. Wulf Pinggera, Dr.

By Dr. Manfred Wick, Prim. Univ.-Prof. Dr. Wulf Pinggera, Dr. Paul Lehmann (auth.)

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It is produced after the proteolytic splitting of approximately 100 amino acids to N-terminus [82]. Methods There is still no reference method for determining the soluble serum transferrin receptor (TfR). On account of the relatively low concentration of soluble serum transferrin receptor in the blood « 10 mg/l or < 100 nMol/I), only sufficiently sensitive measuring methods are suitable for the determination. The TfR determination is rarely carried out as a routine test. Enzyme-linked immunoassay (ErA) is the method most frequently used for scientific investigations [173].

Weight of Vitamin B 12: +++ ReI. mol. weight of Erythropoietin: 445 daltons 1,355 daltons 30,400 daltons 40 Methods all iron determinations in clinical laboratories are performed colorimetric ally, in most cases using routine analyzers. All the colorimetric methods developed for the determination of iron have the following steps in common: - Liberation of the Fe 3+ ions from the transferrin complex by acids or tensides [133, 168]. In some methods, the liberation of Fe 3+ ions by acid is combined with deproteinization after addition of trichloroacetic acid or chloroform.

The radioimmunoassay was introduced in 1956 by Berson and Yalow [12]. Enzyme immunoassays [39, 139], fluorescence immunoassays [66], and luminescence immunoassays [64] followed later as logical developments designed to avoid the disadvantages associated with the use of radioactive materials. The quantitative determination of all direct immunoassays is based on the Heidelberger-Kendall curve [69], which describes the relationship between the antigen concentration and the quantity of precipitate for a constant quantity of antibody.

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