By Gould R.F. (ed.)
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13 shows a growth curve for single-molecule publications which indicates a promising future. Currently, the majority of single-molecule research relies on fluorescence detection. In the near future, other intrinsic properties such as Raman spectra, absorption spectra, electrical properties, magnetic properties, and so on, will be increasingly used to characterize single molecules. 1. Medical Diagnostics An area where single-molecule studies have great potential is medical diagnostics. , antibody antigen).
2002). , 2000). 5 shows a DNA fingerprint from a HindIII digest of λ phage DNA (Habbersett and Jett, 2004). Fluorescence from approximately 1800 fragments is used to construct this histogram. How many were really needed? , 2004). Only two measurements are required to reasonably estimate the mean and m greater than or equal to five does remarkably well for a Gaussian distribution. 6 can be thought of as a graphical representation of the maximum likelihood theorem; that is, the most probable JWDD033-XU February 3, 2007 15:8 Char Count= 0 14 IS ONE ENOUGH?
The reaction time is determined by the number of trials needed for a newly generated random number to meet the specified condition above; the number of trials is then multiplied by dt. In the case for simulations of multiple molecules, data from individual molecules were added together. 1, we choose to start all of the molecules in A in order to simulate a perturbation-jump experiment. Alternatively, when a random start is desired, a uniform random number is compared to one of the probabilities in Eq.