Fibrosis Research: Methods and Protocols by John Varga, David A. Brenner, Sem H. Phan

By John Varga, David A. Brenner, Sem H. Phan

Major investigators assessment the highlights of present fibrosis study and the experimental methodologies used discover the mechanisms that force it. of their dialogue of study methodologies using cultured cells to version numerous points of the fibrotic reaction in vitro, the authors describe the isolation, characterization, and propagation of mesenchymal cells, and spotlight the similarities and variations among equipment which are acceptable for various different types of fibroblasts. techniques for learning collagen gene rules and TGF-b creation also are mentioned, in addition to experimental methodologies using animal versions to check the pathogenesis of fibrosis. The protocols persist with the winning equipment in Molecular medication™ sequence structure, every one delivering step by step laboratory directions, an advent outlining the rules at the back of the strategy, lists of the mandatory apparatus and reagents, and tips about troubleshooting and keeping off identified pitfalls.

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Zwaan, F. , Kluin, P. , Raap, A. , and van der Ploeg, M. (1990) Three sensitive methods for the detection of cytomega- Pulmonary Fibrosis 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 33 lovirus in lung tissue of patients with interstitial pneumonitis. Am. J. Clin. Pathol. 93, 491–494. , and Faber, V. (1988) Computed tomography of the lungs in acquired immunodeficiency syndrome. An early indicator of interstitial pneumonia. Acta Radiol. 29, 641–644. , and Adair, S. (1990) Isolation of measles virus in primary rhesus monkey cells from a child with acute interstitial pneumonia who cytologically had giant-cell pneumonia without a rash.

2002) A rearranged form of Epstein-Barr virus DNA is associated with idiopathic pulmonary fibrosis. Am. J. Respir. Crit. Care Med. 166, 510–513. 27. Tang, Y. , Johnson, J. , Browning, P. , et al. (2003) Herpesvirus DNA is consistently detected in lungs of patients with idiopathic pulmonary fibrosis. J. Clin. Microbiol. 41, 2633–2640. 28. Irving, W. , and Johnston, I. D. (1993) Idiopathic pulmonary fibrosis and hepatitis C virus infection. Am. Rev. Respir. Dis. 148, 1683–1684. 29. , et al. (1996) Incidence of hepatitis C virus infection in Italian patients with idiopathic pulmonary fibrosis.

50. 51. 33 lovirus in lung tissue of patients with interstitial pneumonitis. Am. J. Clin. Pathol. 93, 491–494. , and Faber, V. (1988) Computed tomography of the lungs in acquired immunodeficiency syndrome. An early indicator of interstitial pneumonia. Acta Radiol. 29, 641–644. , and Adair, S. (1990) Isolation of measles virus in primary rhesus monkey cells from a child with acute interstitial pneumonia who cytologically had giant-cell pneumonia without a rash. Am. J. Clin. Pathol. 94, 464–469. Tubbs, R.

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