By Steven D. Schwartzbach, Omar Skalli, Thomas Schikorski
This quantity provides authoritative and state of the art tools and protocols targeting 3 software containers masking the more and more various methodologies used to snapshot chosen proteins and to enquire their functionality by means of gentle and electron microscopy. the 1st software field comprises the improvement of quite a lot of molecular and immunological probes to focus on particular proteins. the second one info using those probes for top solution fluorescence microscopy and the 3rd specializes in purposes for transmission and scanning electron microscopy. Written within the hugely winning Methods in Molecular Biology series layout, chapters contain introductions to their respective themes, lists of the required fabrics and reagents, step by step, effectively reproducible laboratory protocols, and tips about troubleshooting and averting recognized pitfalls hence making sure profitable leads to the extra research of this important field.