Applications of Infrared Spectroscopy in Biochemistry, by Frank Parker

By Frank Parker

This booklet isn't really meant to be a simple textual content in infrared spectroscopy. Many such books exist and i've said them within the textual content. particularly, i've got attempted to discover functions that might be fascinating to various humans: complicated undergraduate chemistry scholars, graduate scholars and learn staff in different disciplines, spectros­ copists, and physicians energetic in study or within the perform of medication. With this target in brain there has been no purpose to have exhaustive insurance of the literature. I may still wish to recognize my use of numerous books and reports, which have been valuable in my look for fabric: G. H. Beaven, E. A. Johnson, H. A. Willis and R. G. 1. Miller, Molecular Spec­ troscopy, Heywood and corporate, Ltd., London, 1961. J. A. Schell guy and Charlotte Schellman, 'The Conformation of Polypeptide Chains in Proteins," within the Proteins, Vol. II, 2d Ed. (H. Neurath, ed.), educational Press, big apple, 1964. R. T. O'Connor, "Application of Infrared Spectrophotometry to Fatty Acid Derivatives," J. Am. Oil Chemists' Soc. 33, 1 (1956). F. L. Kauffman, "Infrared Spectroscopy of fat and Oils," J. Am. Oil Chemists' Soc. 41,4 (1964). W. J. Potts, Jr., Chemical Infrared Spectroscopy, Vol. I, options, Wiley, manhattan, 1963. R. S. Tipson, Infrared Spectroscopy of Carbohydrates, nationwide Bureau of criteria Monograph llO, Washington, D.C., 1968. C. N. R. Rao, Chemical purposes of Infrared Spectroscopy, educational Press, manhattan, 1963.

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Extra info for Applications of Infrared Spectroscopy in Biochemistry, Biology, and Medicine

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Bladon, P. and Eglinton, G. in Physical Methods in Organic Chemistry (J. C. P. ). , San Francisco, 1964. Coleman, I. and Low, M. J. D. Spectrochim. Acta 22, 1293 (1966). Conley, R. T. Infrared Spectroscopy, Allyn and Bacon, Boston, 1966. Conn, G. K. T. and Avery, D. G. Infrared Methods, Academic Press, New York, 1960. Herscher, L. W. in Applied Infrared Spectroscopy (D. N. ), Reinhold, New York, 1966. Herzberg, G. Molecular Spectra and Molecular Structure: Infrared and Raman Spectra of Polyatomie Molecules.

S. N. and Rao, C. N. R. Appl. Spectrosc. Rev. 2, 69 (1968). Pimentel, G. C. and McClellan, A. L. The Hydrogen Bond, W. H. Freeman, San Francisco, 1960. Scarpa, J. , Mueller, D. , and Klotz, I. M. J. Am. Chem. Soc. 89, 6024 (1967). Schejter, A. and George, P. Biochemistry 3,1045 (1964). Smith, L. and Stotz, E. J. Bioi. Chem. 209, 819 (1954). Sophianopoulos, A. , Rhodes, C. , Holcomb, D. , and Van Holde, K. E. J. Bioi. Chem. 237, 1107 (1962). , Timasheff, S. , and Ard, J. S. J. Bioi. Chem. 239, 3051 (1964).

Chicago, Illinois. The powder and several steel balls are put into a stainless steel capsule and then placed in the machine, where they are shaken vigorously for a timed period (timing helps to achieve reproducibility in the spectra). This process yields satisfactory mixing and grinding, and the technique can be used for quantitative work. There are other ways of mixing sample with matrix material. Usually they involve hand grinding with a mortar and pestle or a ball mill. The sample is sometimes ground separately in the presence of a highly volatile solvent to obtain improved distribution of particle size.

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